FOLIKEL ANTRAL OVARIUM PADA PEMBERIAN ANTIBODI MONOKLONA BOVINE ZONA PELUSIDA SEBAGAI KANDIDAT IMUNOKONTRASEPSI
Abstract
Kepadatan penduduk masih merupakan masalah yang penting bagi negara-negara bekembang seperti Indonesia. Di sisi lain penggunaan metode kontrasepsi yang saat ini diandalkan untuk menangani masalah tersebut memiliki bbeberapa kekurangan yang menyababkan upaya menekan jumlah penduduk kurang optimal. Pengembangan metode kontrasepsi dengan target non hormonal seperti penggunan Monoklonal antibodi bovine ZP3 sebagai kandidat bahan imunokontrasepsi perlu dilakukan. Penelitian ini bertujuan mengetahui pengaruh Mab-bZP3 sebagai kandidat bahan immunokontrasepsi terhadap histologi ovarium yaitu jumlah folikel antral yang normal dan atretik. Penelitian True experimental ini dilakukan terhadap 36 hewan coba yang terdiri dari tiga kelompok kontrol dan tiga kelompok perlakuan, masing-masing dianalisa pada hari ke-5, ke-10, dan ke-20 setelah perlakuan. Kelompok kontrol diberikan suntikan Phospat Buffer Saline 50 μl dan kelompok perlakuan diberi suntikan Mab bZP3 sebanyak 50 μl. Hasil penelitian menunjukkan bahwa tidak terdapat perbedaan yang signifikan rata-rata jumlah folikel antral normal (p-value 0,715) maupun folikel antral atretik (p-value 0,604) antara kelompok kontrol dengan perlakuan. Tidak terdapat perbedaan yang signifikan (p-value 0.071) rata-rata jumlah folikel antral atretik antara waktu pemeriksaan pada masing-masing kelompok kontrol dan perlakuan. Namun, terdapat perbedaan yang signifikan (p-value 0.036) rata-rata jumlah folikel antral normal antar waktu pemeriksaan pada masing-masing kelompok kontrol dan perlakuan. Kelompok kontrol yang diperiksa pada hari ke-5, berbeda signifikan hanya dengan kelompok kontrol maupun kelompok perlakuan yang diperiksa pada hari ke-20. Sedangkan rata-rata jumlah folikel antral normal terendah terdapat pada kelompok kontrol yang diperiksa pada hari ke-20, namun tidak berbeda signifikan dengan kelompok perlakuan yang diperiksa pada hari ke-20. Hasil tersebut mengindikasikan bahwa Mab bZP3 sebagai kandidat bahan imunokontrasepsi tidak berpengaruh terhadap perkembangan folikel, dalam hal regulasi apoptosis dan fungsi reseptor FSH pada sel granulosa. Efek kontrseptif bersifat reversible, yang ditunjukkan dengan tidak adanya perbedaan yang signifikan jumlah folikel antral antara kelompok kontrolndan perlakuan pada hari ke-20.
References
2. Soewart, S. and S.B. Sumitro, Perkembangan Kontrasepsi, in Imunokantrasepsi: Konsep-Konsep Dasar dan Bunga Rampai Penelitian2011, Airlangga University Press: Surabaya.
3. Rabe, T., Contraception-Update and Trends. Journal für Reproduktionsmedizin und Endokrinologie-Journal of Reproductive Medicine and Endocrinology, 2007. 4(6): p. 337-357.
4. Sumitro, S.B. and Aulanni’am, Antibodi Zona Pellusida dan Aplikasinya Sebagai Kandidat Imunokontrasepsi Wanita, in Imunokontrasepsi Konsep Dasar dan Bunga Rampai Penelitian2011, Pusat Penerbitan dan Percetakan Unair (AUP): Surabaya. p. 61-66.
5. Sumitro, S.B. and Aulanni’am, Rangkaian Penelitian Penemuan Imunokontrasepsi Wanita Berbasis Molekul Antibodi Hasil Induksi bZP3 dan bZP3DG, in Imunokontrasepsi Konsep Dasar dan Bunga Rampai Penelitian2011, Pusat Penerbitan dan Percetakan Unair (AUP): Surabaya. p. 67-81.
6. Widodo, E. and Aulanni’am, Specificity of antibody bovine zonna pellucidae 3 to rabbit ZP3 based on bZP3 as contraceptive antigens. Indo. J. Chem, 2005. 5(2): p. 182-187.
7. Pantiwati, Y., Passive immunization of anti bZP3 (zona pelusida3) in wistar rat (Rattus Norvegicus) and mouse (Mus Musculus). Media Peternakan, 2012. 1: p. 163-169.
8. Loho, M.F., Cadangan Ovarium 9Ovarian reserve), in Maj Obstet Ginekol Indones2010. p. 43-46.
9. Ireland, J., et al., Antral follicle count reliably predicts number of morphologically healthy oocytes and follicles in ovaries of young adult cattle. Biology of reproduction, 2008. 79(6): p. 1219-1225.
10. Roudebush, W.E., W.J. Kivens, and J.M. Mattke, Biomarkers of ovarian reserve. Biomarker insights, 2008. 3: p. 259.
11. Palma, G.A., et al., Biology and biotechnology of follicle development. The Scientific World Journal, 2012. 2012.
12. Johnson, M. and B. Everitt, Essential reproduction. 2000, Oxford: Blackwell Sciences Ltd. Google Scholar.
13. Chedrese, P.J., Reproductive Endocrinology: A Molecular Approach2009: Springer.
14. McGee, E.A. and A.J. Hsueh, Initial and Cyclic Recruitment of Ovarian Follicles 1. Endocrine reviews, 2000. 21(2): p. 200-214.
15. Avellaira, C., et al., Expression of molecules associated with tissue homeostasis in secretory endometria from untreated women with polycystic ovary syndrome. Human Reproduction, 2006. 21(12): p. 3116-3121.
16. Villarroel, C., et al., Polycystic ovarian morphology in adolescents with regular menstrual cycles is associated with elevated anti-Müllerian hormone. Human Reproduction, 2011. 26(10): p. 2861-2868.
Please find the rights and licenses in Jurnal Ilmiah Bidan (JIB). By submitting the article/manuscript of the article, the author(s) agree with this policy. No specific document sign-off is required.
1. License
The non-commercial use of the article will be governed by the Creative Commons Attribution license as currently displayed on Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.
2. Author(s)' Warranties
The author warrants that the article is original, written by stated author(s), has not been published before, contains no unlawful statements, does not infringe the rights of others, is subject to copyright that is vested exclusively in the author and free of any third party rights, and that any necessary written permissions to quote from other sources have been obtained by the author(s).
3. User/Public Rights
JIB's spirit is to disseminate articles published are as free as possible. Under the Creative Commons license, JIB permits users to copy, distribute, display, and perform the work for non-commercial purposes only. Users will also need to attribute authors and JIB on distributing works in the journal and other media of publications. Unless otherwise stated, the authors are public entities as soon as their articles got published.
4. Rights of Authors
Authors retain all their rights to the published works, such as (but not limited to) the following rights;
- Copyright and other proprietary rights relating to the article, such as patent rights,
- The right to use the substance of the article in own future works, including lectures and books,
- The right to reproduce the article for own purposes,
- The right to self-archive the article (please read out deposit policy),
- The right to enter into separate, additional contractual arrangements for the non-exclusive distribution of the article's published version (e.g., post it to an institutional repository or publish it in a book), with an acknowledgment of its initial publication in this journal (Jurnal Ilmiah Bidan).
5. Co-Authorship
If the article was jointly prepared by more than one author, any authors submitting the manuscript warrants that he/she has been authorized by all co-authors to be agreed on this copyright and license notice (agreement) on their behalf, and agrees to inform his/her co-authors of the terms of this policy. JIB will not be held liable for anything that may arise due to the author(s) internal dispute. JIB will only communicate with the corresponding author.
6. Royalties
Being an open accessed journal and disseminating articles for free under the Creative Commons license term mentioned, author(s) aware that JIB entitles the author(s) to no royalties or other fees.
7. Miscellaneous
JIB will publish the article (or have it published) in the journal if the article’s editorial process is successfully completed. JIB's editors may modify the article to a style of punctuation, spelling, capitalization, referencing and usage that deems appropriate. The author acknowledges that the article may be published so that it will be publicly accessible and such access will be free of charge for the readers as mentioned in point 3.
